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object --+ | type --+ | RestrictionType --+ | AbstractCut --+ | TwoCuts
Implement the methods specific to the enzymes that cut the DNA twice
Correspond to ncuts values of 4 in emboss_e.###
Internal use only. Not meant to be instantiated.
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bool |
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bool |
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int |
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generator of int |
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tuple |
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for internal use only. location is an integer corresponding to the location of the match for the enzyme pattern in the sequence. _modify returns the real place where the enzyme will cut. example : EcoRI pattern : GAATTC EcoRI will cut after the G. so in the sequence : ______ GAATACACGGAATTCGA | 10 dna.finditer(GAATTC, 6) will return 10 as G is the 10th base EcoRI cut after the G so : EcoRI._modify(10) -> 11. if the enzyme cut twice _modify will returns two integer corresponding to each cutting site.
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for internal use only. as _modify for site situated on the antiparallel strand when the enzyme is not palindromic
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