Experiment
Experiment Design
Type of Experiment:
Comparison of PBMCs from
3 resistant and 3 multiple
time infected children during the first 3 years of
follow-up of our cohort
study.
Experimental
Factors:
02-29-199
02-29-3047
02-29-3182
02-29-3422
02-29-3433
Number
of hybridizations performed:
Quality control steps
taken:
None. No duplicates.
URLS of supplemental
websites:
Samples Used, Extraction Preparation, and Labeling
Origin of the
biological sample and its characteristics:
Child ID Age Sex Infection
Status
199 8 years Female Infected
3047 8 years Male Infected
3433 8
years Male Infected
1291 6 years Female Not Infected
3182 8 years
Female Not Infected
3422 71/2 years Male Not
Infected
Manipulation of the samples and the protocols
used:
Manipulation of the samples and the protocols used: We have used
TRIZOL
reagents (Cat No.: 15596-026) of LIFE TECHNOLOGIES to extract RNA
from
the PBMC. The PBMC were not grown in vitro with soluble amebic extract
or
anything.
External controls (spikes)
added:
No
Measurement Data and Specifications
How the data is analyzed and
transformed:
Dchip